Colorimetric Assay to Quantify Macromolecule Diffusion across Endothelial Monolayers
نویسندگان
چکیده
منابع مشابه
Colorimetric assay to quantify macromolecule diffusion across endothelial monolayers.
Endothelial "capillary leak", the loss of vascular integrity in response to noxious stimuli, is characterized by extravasation of protein-richfluidfrom capillary lumen into surrounding tissue interstitium. This increase in vascular permeability, in response to inflammatory mediators, correlates with endothelial cell contraction and the formation of intercellular gaps within the monolayer. Howev...
متن کاملATP reduces macromolecule permeability of endothelial monolayers despite increasing [ Ca
Noll, T., H. Hölschermann, K. Koprek, D. Gündüz, W. Haberbosch, H. Tillmanns, and H. M. Piper. ATP reduces macromolecule permeability of endothelial monolayers despite increasing [Ca]i. Am. J. Physiol. 276 (Heart Circ. Physiol. 45): H1892–H1901, 1999.—We investigated the relationship between theATP-evoked rise of cytosolic Ca21 concentration ([Ca]i) and barrier function in porcine aortic endoth...
متن کاملA colorimetric reaction to quantify fluid mixing
We found the colorimetric reaction of Tiron (1,2-dihydroxybenzene-3,5-disulfonic acid) and molybdate suitable for optical quantification of chemical reaction during fluid–fluid mixing in laboratory chambers. This reaction consists of two colorless reagents that mix to rapidly form colored, stable, soluble products. These products can be digitally imaged and quantified using light absorbance to ...
متن کاملHistamine type I receptor occupancy increases endothelial cytosolic calcium, reduces F-actin, and promotes albumin diffusion across cultured endothelial monolayers
Considerable evidence suggests that Ca2+ modulates endothelial cell metabolic and morphologic responses to mediators of inflammation. We have used the fluorescent Ca2+ indicator, quin2, to monitor endothelial cell cytosolic free Ca2+, [Ca2+]i, in cultured human umbilical vein endothelial cells. Histamine stimulated an increase in [Ca2+]i from a resting level of 111 +/- 4 nM (mean +/- SEM, n = 1...
متن کاملATP reduces macromolecule permeability of endothelial monolayers despite increasing [Ca2+]i.
We investigated the relationship between the ATP-evoked rise of cytosolic Ca2+concentration ([Ca2+]i) and barrier function in porcine aortic endothelial monolayers. ATP (0.01-100 μM) induced a transient rise of [Ca2+]iand reduced permeability in a concentration-dependent manner. In contrast, the Ca2+ ionophore ionomycin (1 μM) elicited a rise in [Ca2+]icomparable to that induced by ATP (10 μM),...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2001
ISSN: 0736-6205,1940-9818
DOI: 10.2144/01313st02